I Semester(Genetics)
(Paper-I:Fundamentals of cellBiology)
1. Scope of genetics (genetics)
2. Microscopy
(Paper-I:Fundamentals of cellBiology)
1. Scope of genetics (genetics)
2. Microscopy
- Use of Microscopes in cytology
- Principles, Magnification, Resolving power N handling of different Microscopes compound, Dark field, Bright field, phase contrast, fluorescent N electron microscope
3. Model Organisms: Life Cycle of
- Viruses: TMV
- Bacteriophage- lambda phage
- Bacteria-E.Colin
- Neurospora
- Coenorhabditis elegans
- Drosophila
- Mosquitoes(culex)
- Bombyx Moro
- Arabidopsis thaliana
4. Ultra structure of cell and cell organelles
- Cell Theory: prokaryotic cell and Eukaryotic cell
- Cell wall: Ultra structure and Function, Extracellular matrix, cytoskeletal structure-Actin and Microtubules.
- Plasma membrane: chemical composition, ultra structure-Fluid mosaic Model, Functions-Osmosis, phagocytosis, pinocytosis, Active Transport.
- Microvilli and Desmosomes.
- Ultra structure, chemical composition function of cytoplasmic organelles, Endoplasmic reticulum, Ribosomes, Centrosomes, lysosomes, Golgi complex and peroxisomes.
- Mitochondria: Ultrastructure, Chemical composition, enzyme and coenzyme. Function, kreb's cycle, Electron-transport system and Oxidative phosphorylation.
- Plastids: Types, Ultra structure of chloroplast and role in photosysthesis.
- Nucleus: Morphology, Nuclear - envelope, Nucleolus, Nucleoplasm chromatin.
5. Cell cycle and cell division
- Cell cycle: -G1phase
- -S phase
- - G2phase
- - M phase
- Mitosis: Essentials of mitosis, Mitotic apparatus, its structure and chemistry, Mitotic phases, Cytokinesis, Mitotic blockage and stimulation of cell division, significance of Mitosis.
- Meiosis: Stages, synaptonemal complex, crossing over and chiasm a formation, significance of Meiosis.
- Cell senescence and cell Death (APOPTOSIS): Death of specified cell, programmed cell death, mechanism of cell death and significance.
Practical-I (video experiment)
1. Microscopy:
Handling of dissection, stereo and compound microscope.
2. Model Organism and their significance in Genetic studies:
- Virus TMV(tobacco leaves)
- Bacteriophage- lambda phage
- Bacteria E.coli (slide)
- Neurospora
- Coenorhabditis elegans
- Drosophila melanogaster
- Mosquito (culex)
- Bombyx Maori
- Arabidopsis thaliana
3. Staining techniques:
- RNA and DNA -- Methyl green and pyronin
- Mitochondria - Janus green
- Lactobacillus and E.Colo -- Gram Staining
4. : Mitotic stages in slide
5. Onion tip for mitosis.
http://www.dnatranscription.blogspot.in/2014/06/hshadap.transcription
of DNA into mRNA
CLICK REFERENCE
Genetics
Paper 6 Human
Genetics
Paper8 Genetics
(Applied Genetics)
BIOCHEMISTRY
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